Regions Of Theworld Research Articles

Targeting Cysteine Proteases and their Inhibitors to Combat Trypanosomiasis.

Trypanosomiasis, caused by protozoan parasites of the Trypanosoma genus, remains a significant health burden in several regions of the world. Cysteine proteases play a crucial role in the pathogenesis of Trypanosoma parasites and have emerged as potential therapeutic targets for the development of novel antiparasitic drugs. This review article aims to provide a comprehensive overview of the role of cysteine proteases in trypanosomiasis and their potential as therapeutic targets. We discuss the biological significance of cysteine proteases in Trypanosoma parasites and their involvement in essential processes, such as host immune evasion, cell invasion, and nutrient acquisition. A comprehensive literature search was conducted to identify relevant studies and research articles on the role of cysteine proteases and their inhibitors in trypanosomiasis. The selected studies were critically analyzed to extract key findings and provide a comprehensive overview of the topic. Cysteine proteases, such as cruzipain, TbCatB and TbCatL, have been identified as promising therapeutic targets due to their essential roles in Trypanosoma pathogenesis. Several small molecule inhibitors and peptidomimetics have been developed to target these proteases and have shown promising activity in preclinical studies. Targeting cysteine proteases and their inhibitors holds great potential for the development of novel antiparasitic drugs against trypanosomiasis. The identification of potent and selective cysteine protease inhibitors could significantly contribute to the combat against trypanosomiasis and improve the prospects for the treatment of this neglected tropical disease.

Global, regional, and national burden of pancreatitis in older adults, 1990–2019: A systematic analysis for the global burden of disease study 2019

BackgroundTo describe the past, present and future burden of pancreatitis in older adults, and to explore cross-national inequalities across socio-demographic index (SDI). MethodsData on pancreatitis in older adults, including mortality and disability-adjusted life years (DALYs) rates, were collected from the Global Burden of Disease (GBD) 2019 study. Temporal trends were measured using joinpoint analyses and predicted using a Bayesian age-period-cohort model. Additionally, the unequal distribution of the burden of pancreatitis in older adults was quantified. ResultsFrom 1990 to 2019, the number of deaths and DALYs due to pancreatitis in older adults has been increasing annually. However, in most regions of the world, age-standardized death rates (ASDR) and age-standardized DALYs rates have been declining. The burden of pancreatitis in older adults was highest in low SDI region, primarily affecting the population aged 65–74, with a greater burden on males than females. Furthermore, from 1990 to 2019, absolute and relative cross-national inequalities in pancreatitis among older adults have remained largely unchanged. It is projected that in the next 11 years, the number of deaths in older adults due to pancreatitis will continue to increase, but the ASDR is expected to decline. ConclusionOver the past 30 years, the ASDR and age-standardized DALYs rate of pancreatitis in older adults have shown a decline globally, but the absolute burden continues to increase. Cross-national health inequalities persist. Therefore, it is necessary to develop targeted intervention measures and enhance awareness among this vulnerable population regarding the risk factors associated with pancreatitis.

Scaling use of the rust fungus Puccinia punctiformis for biological control of Canada thistle (Cirsium arvense (L.) Scop.): First report on a U.S. Statewide effort

Canada thistle (Cirsium arvense (L.) Scop., CT) is one of the worst weeds threatening temperate regions of the world. A host-specific rust fungus, Puccinia punctiformis (F. Strauss) Rohl., is known to cause systemic disease of CT, ultimately killing individuals and reducing stand densities. In 2013, it was demonstrated that fall inoculation of rosettes with coarsely ground leaves bearing P. punctiformis telia can successfully initiate epiphytotics. In the same year, a cooperative project between the Colorado Department of Agriculture and United States Department of Agriculture was initiated, in which CT patches across the state of Colorado (USA) were inoculated and tracked over subsequent years for changes in stem density. Here, we report our findings from 8 years (2014–2021) of monitoring effort. At most sites (N = 87), CT stem densities declined, from a mean (±SE) of 87.9 (±6.5) stems to 44.7 (±4.2). These declines however were spatially-autocorrelated, and likely attributable to local growing conditions, as mean annual daily maximum temperature and standard deviation of elevation, as well as climatic conditions around the times of both treatment and monitoring, were found to be important predictors of CT decline. Further, we observed that the amount of inoculum deployed, timing since last release, and method in which it was spread locally at a site were also associated with the magnitude of CT stem decline. These results are indicative of the value of P. punctiformis as a CT biological control agent.

An exhaustive review on pharmacological potential, bioactive components and formulations of Moringa oleifera

Moringa oleifera, native to India, grows in the tropical and subtropical regions of the world. It is commonly known as ‘drumstick tree’ or ‘horseradish tree’ and has gained attention in recent years for its nutritional and medicinal properties. This review article aims to provide a comprehensive overview of the current scientific understanding of Moringa oleifera. The review covers the plant's botany, nutritional composition, and phytochemical content. It also discusses the plant's pharmacological and medicinal properties, including its anti-inflammatory, anti-cancer, anti-diabetic, anti-viral and anti-microbial activities. Additionally, the review examines the potential applications of Moringa oleifera in food and agriculture, as well as its industrial uses, such as in biofuel production. Summarizing, this review article provides a comprehensive and scientific overview of Moringa oleifera, highlighting its potential as a valuable plant resource with numerous applications and benefits for human health and well-being. The review article also identifies areas for future research to further explore the plant's potential and unlock its full potential as a natural resource.

Identity, pathogenicity and genetic diversity of Lasiodiplodia associated with stem-end rot of avocado in China.

Stem-end rot (SER) causes brown necrotic lesions in the pulp near the base of the fruit pedicel and is one of the most devastating postharvest diseases of avocados in all avocado growing regions of the world. China's avocado industry is growing very rapidly, and the planting area is expanding, but little is known about the pathogens and genetic diversity of avocado SER. To determine the causal agents of SER, avocado fruits were sampled from the main avocado-producing areas in China during 2020 and 2021. Fungal isolates were obtained from SER symptomatic avocado fruits and identified by morphology combined with phylogenetic analysis of internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α) and β-tubulin (TUB2) gene sequences. All 101 isolates belonged to Lasiodiplodia spp., and four Lasiodiplodia species were identified, namely L. pseudotheobromae (59.41%), L. theobromae (24.75%), L. mahajangana (7.92%), L. euphorbiaceicola (1.98%), and six others are classified as Lasiodiplodia sp. (5.94%). There were only slight morphological differences in colonies and conidia of these four species of Lasiodiplodia. The pathogenicity tests showed symptoms of SER, and the 92.08% of the isolates exhibited a high level of virulence on avocado (disease index > 70), related to the disease severity on avocado fruit. All tested isolates grew well under conditions from 23 to 33℃. There was a significant difference in mycelial growth between the four species of Lasiodiplodia after treatment with high temperature or low temperature. L. pseudotheobromae growth was the fastest at 13 to 18℃, but was the lowest at 38℃ (P < 0.05). Red pigment could be produced by all tested isolates after culturing for 7 days at 38℃. The mycelial growth rate was the fastest on PDA medium, and the slowest on OMA medium but promoted spore formation (P < 0.05). In addition, was determined the genetic diversity of Lasiodiplodia pathogenic species associated with SER collected from avocado, mango, guava and soursop fruits was determined. A total of 74 isolates were clustered into 4 main ISSR groups by unweighted pair-group method with arithmetic mean (UPGMA) analysis, and the classification of this group was related to the host. Extensive diversity was detected in the Lasiodiplodia populations. The diverse geographical origins and host species significantly influenced the population differentiation, and most of the genetic variation occurred within populations (P < 0.001). This is the first study to identify the major pathogens of avocado SER in China and to survey their occurrence, pathogenicity and include a comparative analysis of genetic diversity with Lasiodiplodia spp. causing SER on other fruit hosts. Collectively, the Lasiodiplodia species complex affecting avocado showed high pathogenicity and diversity, while L. pseudotheobromae was the most frequently isolated species in China. The results of this study provide insights into the aspects of epidemic of SER disease caused by Lasiodiplodia species, which will help in developing strategies for the management and control of stem end-rot in avocado.

Optimizing nutrient solution for vegetative growth of Dendrobium Tubtim Siam and Phalaenopsis Taisuco Swan through plant tissue nutrient balance estimation

BackgroundOrchids are grown without soil in many regions of the world, but there is a lack of studies to define the balanced and adequate nutrient solution for their cultivation, mainly in the vegetative growth phase. Therefore, this paper aims to evaluate the optimal concentration of the nutrient solution based on the proposal by Hoagland and Arnon (1950) in the vegetative growth phase capable of increasing the nutrient contents, growth, and dry matter production of Dendrobium Tubtim Siam and Phalaenopsis Taisuco Swan. In addition, this paper aims to estimate a new nutrient solution from the optimal nutrient contents in the dry matter of these orchid species to be used in the vegetative growth phase.ResultsNutrient contents, growth, and dry matter production increased as the nutrient solution concentration increased up to an average concentration of 62 and 77% for D. Tubtim Siam and P. Taisuco Swan, respectively. We found that the Hoagland and Arnon solution presented a group of nutrients with concentrations above the requirement for P. Taisuco Swan (nitrogen, phosphor, calcium, and sulfur) and D. Tubtim Siam (phosphor, calcium, magnesium, and sulfur), while other nutrients in the solution did not meet the nutritional demand of these orchid species, inducing nutritional imbalance in the vegetative growth phase.ConclusionWe conclude that using a balanced nutrient solution created specifically for each orchid species in vegetative growth might favor their sustainable cultivation by optimizing the use of nutrients in the growing medium.

Quantification of flavonoids, minerals, and pigments present in "Nanicão" bananas during the ripening process.

Banana is one of the most consumed and popular fruits in all regions of the world, being cultivated mainly in tropical countries. It is not only a rich source of vitamins A, C, and B, calcium, iron, potassium, phosphorus, and other vitamins and nutrients, but it also contains several types of antioxidants with high nutritional value. In this context, the current study aimed to quantify the content of ascorbic acid, flavonoids, pigments, and minerals present in "Nanicão" bananas during the ripening process. As demonstrated, the level of flavonoids was higher in ripe and overripe fruits, whereas the mineral composition was high only at ripening stage 4 (more yellow than green) a stage that should be prioritized when recommending fruit consumption to the population deficient in these minerals. Regarding pigments, there was a reduction in chlorophylls a and b and an increase in carotenoids and anthocyanins in peels and pulps. PRACTICAL APPLICATION: Flavonoids are phenolic, bioactive compounds with proven antioxidant and anti-inflammatory activity and products of the plant's secondary metabolism. The degradation of chlorophylls and synthesis of carotenoids and anthocyanins, and as a consequence of the latter pigment, the increase in flavonoids in the pulp was evident during the monitoring of ripening, mainly in the fruit peels in relation to pigments. Minerals are essential elements, the main ones provided in balanced diets and important for dietary and nutritional health.

Interannual variations in the seasonal cycle of extreme precipitation in Germany and the response to climate change

Abstract. Annual maxima of daily precipitation sums can be typically described well with a stationary generalized extreme value (GEV) distribution. In many regions of the world, such a description does also work well for monthly maxima for a given month of the year. However, the description of seasonal and interannual variations requires the use of non-stationary models. Therefore, in this paper we propose a non-stationary modeling strategy applied to long time series from rain gauges in Germany. Seasonal variations in the GEV parameters are modeled with a series of harmonic functions and interannual variations with higher-order orthogonal polynomials. By including interactions between the terms, we allow for the seasonal cycle to change with time. Frequently, the shape parameter ξ of the GEV is estimated as a constant value also in otherwise instationary models. Here, we allow for seasonal–interannual variations and find that this is beneficial. A suitable model for each time series is selected with a stepwise forward regression method using the Bayesian information criterion (BIC). A cross-validated verification with the quantile skill score (QSS) and its decomposition reveals a performance gain of seasonally–interannually varying return levels with respect to a model allowing for seasonal variations only. Some evidence can be found that the impact of climate change on extreme precipitation in Germany can be detected, whereas changes are regionally very different. In general, an increase in return levels is more prevalent than a decrease. The median of the extreme precipitation distribution (2-year return level) generally increases during spring and autumn and is shifted to later times in the year; heavy precipitation (100-year return level) rises mainly in summer and occurs earlier in the year.

Molecular diversity of black corals from the Saudi Arabian Red Sea: a first assessment

Black corals occur as part of benthic assemblages from shallow to deep waters in all oceans. Despite the importance in many benthic ecosystems, where these act as biodiversity aggregators, antipatharians remain poorly studied, with 75% of the known species occurring below recreational SCUBA diving depth limits. Currently, information regarding the diversity and evolutionary history is limited, with most studies focusing on Hawaii and the South Pacific Ocean. Other regions of the world have received less attention, such as the Red Sea, where only two black coral families and four genera have been recorded. We provide the first analysis of the molecular diversity of black corals in the eastern Gulf of Aqaba and the northern and central Saudi Arabian Red Sea, based on a dataset of 161 antipatharian colonies collected down to 627 m deep. Based on specimen morphology, we ascribed our material to 11 genera belonging to 4 of the 7 known Antipatharia families, i.e. Antipathidae, Aphanipathidae, Myriopathidae and Schizopathidae. The genus level phylogeny of three intergenic mitochondrial regions, the trnW-IGR-nad2 (IgrW), nad5-IGR-nad1 (IgrN) and cox3-IGR-cox1 was reconstructed including previously published material. Overall, we recovered six molecular clades that included exclusively Red Sea sequences, with the highest diversity occurring at mesophotic depths. This study highlights that diversity of black corals in the Red Sea is much higher than previously known, with seven new generic records, suggesting that this basin may be a hotspot for antipatharian diversity as is known for other taxa. Our results recovered unresolved relationships within the order at the familial and generic levels. This emphasises the urgent need for an integration of genomic-wide data with a re-examination of informative morphological features necessary to revise the systematics of the order at all taxonomic levels.

Analysis of Favipiravir Adverse Drug Reactions during COVID-19 Pandemic: A Retrospective Study Based on Iraqi Pharmacovigilance Center Database

The “severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)” was the reason behind the recent pandemic “COVID-19” that started from Wuhan, china, and rapidly spread to many regions of the world. Research in Drug repurposing processes to treat this novel coronavirus involved many medications, one of the most discussed is Favipiravir. The objective of the current work was aimed towards Examining the cause, severity, preventability, predictability, and outcome of favipiravir-associated adverse events that had been reported in Iraq. In terms of adverse drug responses, "Gastrointestinal disorders" accounted for the majority (57.4%), followed by "Cardiac disorders" (35.2%), and "Investigations" (abnormal lab test results) (13%). The causality of these reactions Is majorly “Possible” (62%). Severity level 1 (40.9%) and 2 (41.8%). Ninety-nine percent of the ADRs are expected. The majority of the ADRs are non-Preventable (76.3%). The main outcome is Recovered / Resolved (44.5%). About (50%) of the ADRs were serious.

Opportunities and challenges for precipitation forcing data in post‐wildfire hydrologic modeling applications

AbstractThe frequency and extent of wildfires have increased in recent decades with immediate and cascading effects on water availability in many regions of the world. Precipitation is used as primary input to hydrologic models and is a critical driver of post‐wildfire hydrologic hazards including debris flows, flash floods, water‐quality effects, and reservoir sedimentation. These models are valuable tools for understanding the hydrologic response to wildfire but require accurate precipitation data at suitable spatial and temporal resolutions. Wildfires often occur in data‐sparse, headwater catchments in complex terrain, and post‐wildfire hydrologic effects are particularly sensitive to high‐intensity, short‐duration precipitation events, which are highly variable and difficult to measure or estimate. Therefore, the assessment and prediction of wildfire‐induced changes to watershed hydrology, including the associated effects on ecosystems and communities, are complicated by uncertainty in precipitation data. When direct measurements of precipitation are not available, datasets of indirect measurements or estimates are often used. Choosing the most appropriate precipitation dataset can be difficult as different datasets have unique trade‐offs in terms of spatial and temporal accuracy, resolution, and completeness. Here, we outline the challenges and opportunities associated with different precipitation datasets as they apply to post‐wildfire hydrologic models and modeling objectives. We highlight the need for expanded precipitation gage deployment in wildfire‐prone areas and discuss potential opportunities for future research and the integration of precipitation data from disparate sources into a common hydrologic modeling framework.This article is categorized under: Science of Water &gt; Hydrological Processes Science of Water &gt; Methods Science of Water &gt; Water and Environmental Change

Medicinal utilization and nutritional properties of drumstick (Moringa oleifera)—A comprehensive review

AbstractThe tropical and subtropical regions of the world support the growth of the Indian plant Moringa oleifera. It usually goes by the name drumstick tree or horseradish tree and thrives in warm climates. The leaves of the M. oleifera tree are now frequently used as nutrients and nutraceuticals due to their availability of various minerals. While having only very minor antinutritional effects, the leaves are abundant in many beneficial compounds. A recent review of the bioactive components and activity of moringa leaves has focused on both in vivo and in vitro studies. Drumstick leaves have antidiabetic qualities, anti‐inflammatory, anticancer, and antibacterial qualities among other health benefits. Phytochemicals, in addition to minerals and vitamins, are abundant in this vegetable. The majority of these effects, according to a review in the literature, are mostly brought on by the presence of carotenoids, glucosinolates, and phytochemicals. As a value‐added component in the production of wholesome meals, moringa is becoming more popular. Despite extensive research into locating and quantifying these advantageous elements in drumstick leaves, bioavailability and bioaccessibility studies were carried out. Beneficial photochemicals are absorbed and digested through incredibly intricate processes that involve several physicochemical and physiological interactions. Therefore, the biological impact of food may be attributed to its various metabolites that can access particular areas of action rather than its original substances. This body of literature offers the most recent findings in scientific research on the bioavailability, health advantages, nutritional profiles, and bioactive activities of moringa leaves as they relate to their use in a range of food products. Drumsticks are frequently used as a food element that promotes health because of their potent protection against a variety of ailments and the presence of environmental pollutants.

Covid-19 vaccination and stock markets volatility around the world. Evidence from wavelet analysis

ABSTRACT The impact of COVID-19 vaccination on stock markets volatility has been the focus of academics in the aftermath of the pandemic. We use wavelet coherence analysis on the daily stock markets for 74 countries, covering all regions of the world to test relationship between vaccination and stock markets. Our results show the positive effect of vaccination against COVID-19 for all stock markets, while the effects are stronger for developed countries and weaker for emerging countries. Furthermore, effect is present in short-medium term for emerging economies, while for developed countries the effect is present also in the long-term.

Towards a South American High Impact Weather Reports Database

Abstract Despite Southern South America being recognized as a hotspot for deep convective storms, little is known about the socio-environmental impacts of high impact weather (HIW) events. Although there have been past efforts to collect severe weather reports in the region, they have been highly fragmented among and within countries, sharing no common protocol, and limited to a particular phenomenon, a very specific region or a short period of time. There is a pressing need for a more comprehensive understanding of the present risks linked to HIW events, specifically deep convective storms, on a global scale as well as their variability and potential future evolution in the context of climate change. A database of high-quality and systematic HIW reports and associated socio-environmental impacts is essential to understand the regional atmospheric conditions leading to hazardous weather, to quantify its predictability and to build robust early warning systems. To tackle this problem and following successful initiatives in other regions of the world, researchers, national weather service members, and weather enthusiasts from Argentina, Brazil, Chile, Paraguay and Uruguay have embarked on a multi-national collaboration to generate a standardized database of reports of HIW events principally associated with convective storms and their socio-environmental impacts in South America. The goal of this paper is to describe this unprecedented initiative over the region, to summarize first results and to discuss the potential applications of this collaboration.

[Toward Cultural Scripts of Trauma Sequelae].

The concept of cultural scripts has proven to be very useful for describing depression-like psychological complaints in different cultural regions of the world. The article presents the galaxy model and its implementation in the model of cultural scripts theoretically for the first time and connects these with cultural psychological value research. A new, extensive series of research projects aims to investigate post-trauma -related psychological complaints in various regions of the world. The work in two countries/regions began with focus groups in which elements of cultural scripts were collected by traumatized clients and/or by (therapeutic) experts. For this purpose, a theory-based framework of possible post-traumatic script elements was provided. In Switzerland, focus groups were conducted with patients and experts. Focus groups were conducted with genocide survivors and other trauma victims in Rwanda and three East African countries. The evaluation was semi-quantitative. In Switzerland, around 50 symptoms and changes were mentioned as script elements (e. g. still having to function). Approximately 100 symptoms and changes were mentioned as script elements in East Africa (e. g. loss of dignity). The first temporal connections (i. e. strong scripts) were found for the Swiss scripts (e. g. self-devaluation - urge to function). In the East African study, cultural value orientations underlying the script groupings were also assessed (e. g. community reputation). The illustrative results presented here demonstrate the galaxy model and the suitability of the cultural script concept for recording the effects of trauma. There are differences and similarities in the two world regions examined so far. Further steps at the two study sites will be the temporal connections and the relationships to value orientations. The studies will be started at the other study sites in the following years.

Abstract LB336: The African Cancer Atlas: Leveraging African data to diversify precision oncology

Abstract Africa has some of the highest age adjusted cancer mortality rates globally and cancer incidence in Africa is expected to double by 2040. Despite this disproportionate burden of disease, African cancer patients are woefully understudied along the cancer continuum. People of African descent are significantly underrepresented in cancer research, accounting for less than 3% of globally available genomic data and are often the least well served by advances in precision medicine.To address this gap, we have initiated the African Cancer Atlas project. This is an Africa-centred approach to overcoming cancer inequities - providing much needed biological and clinical data, but also seeking to identify and address the social determinants of inequities both within the continent and between high and low-middle income countries (LMIC). We have established an oncology partner network across 9 geographically dispersed African countries (Senegal, Côte d’Ivoire, Ghana, Nigeria, Kenya, Uganda, Zimbabwe, South Africa, and Tunisia). We have developed LMIC-specific standardised protocols and best practices for sample collection and processing across multiple sites and continue to build a well-annotated bank of tissue, plasma, bone marrow and PBMC from individuals with breast, prostate, gastrointestinal cancers, liver, urinogenital and pediatric cancers that will be unrivalled in its diversity. So far, 1800 cancer samples have been collected and banked. We have also built a unique cloud-based clinical data management platform to facilitate the collection of longitudinal clinical metadata into a unified clinico-demographic database. We have initiated extensive genomic and molecular profiling of African-specific cancers using whole exome/genome sequencing (WES/WGS) of paired germline and tumour samples as well as RNA- sequencing of tumors. We have so far completed the first 200 WES, representing the first cancer samples sequenced entirely on the African continent. Ongoing work is investigating how population-specific genetic variation contributes to cancer risk, identifying driver mutations, somatic copy number alterations (SCNAs) and mutational signatures that can point to disease-relevant environmental exposures or impaired core biological pathways. Comparative analysis with existing cancer genomics cohorts will determine the prevalence of known hotspot mutations, cancer drivers and or common SCNAs in African cancer genomes. By comparing with available data for African Americans and other diasporan Africans, we will provide better understanding of cancer aetiology related to African ancestry applicable to the broader African diasporic communities in other regions of the world. We are also collecting data to assess socio-economic and health system factors affecting access to cancer care and survival across all study sites. Socio-demographic data collection is integrated with tissue/biodata collection creating digital longitudinal hospital-based cohorts. We will develop systems that integrate genomics, molecular demographics and clinical data with machine learning and a publicly accessible portal for data access and analytics will be available for the broader scientific community. Ultimately, this project will contribute towards better understanding of cancer among people of African descent and make precision oncology more effective for diverse populations. Citation Format: Yaw Bediako, Joyce Ngoi, Ruth Kiome, Seth Agyemang, Stephen Darkoh, Audrey Lemacon, Aida Manu, Frank Onyambu, Kanny Diallo, Lily Paemka, David Hutchful, Emmanuella Amoako, Abdoulaye Diallo. The African Cancer Atlas: Leveraging African data to diversify precision oncology [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB336.

Lyme borreliosis in a French expatriate in India.

Lyme borreliosis is known to be endemic in temperate countries of the Northern hemisphere but is rarely reported in other regions of the world. Here, we describe a rare diagnosis in a traveller returning from India, who presented with two erythema migrans.

The prevalence and demographic associations of headache in the adult population of Benin: a cross-sectional population-based study

BackgroundThe Global Burden of Disease (GBD) study is increasingly well informed with regard to headache disorders, but sub-Saharan Africa (SSA) remains one of the large regions of the world with limited data directly derived from population-based studies. The Global Campaign against Headache has conducted three studies in this region: Ethiopia in the east, Zambia in the south and Cameroon in Central SSA. Here we report a similar study in Benin, the first from West SSA.MethodsWe used the same methods and questionnaire, applying cluster-randomized sampling in three regions of the country, randomly selecting households in each region, visiting these unannounced and randomly selecting one adult member (aged 18–65 years) of each household. The HARDSHIP structured questionnaire, translated into Central African French, was administered face-to-face by trained interviewers. Demographic enquiry was followed by diagnostic questions based on ICHD-3 criteria.ResultsFrom 2,550 households with eligible members, we recruited 2,400 participants (participating proportion 94.1%). Headache ever was reported by almost all (95.2%), this being the lifetime prevalence. Headache in the last year was reported by 74.9%. Age-, gender- and habitation-adjusted estimates of 1-year prevalence were 72.9% for all headache, 21.2% for migraine (including definite and probable), 43.1% for TTH (also including definite and probable), 4.5% for probable medication-overuse (pMOH) and 3.1% for other headache on ≥ 15 days/month. One-day (point) prevalence of headache was 14.8% according to reported headache on the day preceding interview.ConclusionsOverall, these findings are evidence that headache disorders are very common in Benin, a low-income country. The prevalence of pMOH, well above the estimated global mean of 1–2%, is evidence that poverty is not a bar to medication overuse. The findings are very much the same as those in a similar study in its near neighbour, Cameroon. With regard to migraine, they are reasonably in accord with two of three earlier studies in selected Beninese populations, which did not take account of probable migraine. This study adds to the hitherto limited knowledge of headache in SSA.

The Potential of Traditional Medicine in Combating COVID-19: A Systematic Review and Meta-Analysis

Objectives: The aim of the study was to review the existing data on traditional medicine in reducing the symptoms of COVID-19 patients with mild, moderate, and severe symptoms. We also investigated the adverse impact, patient outcome, source, and mode of action of traditional medicine. A brief comparison was made on adverse impacts and symptom alleviation of the commercially available drugs as well. Methods: We utilized PubMed, Scopus, WHO (GHL), and VHL in order to choose the eligible studies for the systematic review between July and August 2022. From a total of 12,263 studies, after a series of screening, 285 articles were identified in the final sample. The methodological evaluation was carried out accordingly. Results: There is a growing literature on the usage of traditional medicine for COVID-19. The majority of the studies have shown positive outcomes even though they were not carried out at diverse locations around the world. We identified that the majority (17.4%) of the traditional medicine was derived from plants. The average time in the disappearance of the symptoms was 8.8 days, whereas the disappearance of symptoms using conventional drugs (Remdesevir, Ivermectin, Tocilizumab, Baricitinib, Famotidine, Ensitrelvir and Molnupiravir) was around 12 days. The mode of action of traditional medicine was mostly the reduction of viral load (50%). In terms of the severity of the patients, most of the patients (37.5%) had mild symptoms. We also found that no major adverse impact was reported on administering the traditional medicine among the patients. Further, the majority of the study was carried out in the Asian region, mostly in China. Conclusion: Apart from expanding the study to different regions of the world, to improve the quality of data, larger-scale clinical studies in the Asian region are required.

Renewable Energy Potential: Second-Generation Biomass as Feedstock for Bioethanol Production.

Biofuels are clean and renewable energy resources gaining increased attention as a potential replacement for non-renewable petroleum-based fuels. They are derived from biomass that could either be animal-based or belong to any of the three generations of plant biomass (agricultural crops, lignocellulosic materials, or algae). Over 130 studies including experimental research, case studies, literature reviews, and website publications related to bioethanol production were evaluated; different methods and techniques have been tested by scientists and researchers in this field, and the most optimal conditions have been adopted for the generation of biofuels from biomass. This has ultimately led to a subsequent scale-up of procedures and the establishment of pilot, demo, and large-scale plants/biorefineries in some regions of the world. Nevertheless, there are still challenges associated with the production of bioethanol from lignocellulosic biomass, such as recalcitrance of the cell wall, multiple pretreatment steps, prolonged hydrolysis time, degradation product formation, cost, etc., which have impeded the implementation of its large-scale production, which needs to be addressed. This review gives an overview of biomass and bioenergy, the structure and composition of lignocellulosic biomass, biofuel classification, bioethanol as an energy source, bioethanol production processes, different pretreatment and hydrolysis techniques, inhibitory product formation, fermentation strategies/process, the microorganisms used for fermentation, distillation, legislation in support of advanced biofuel, and industrial projects on advanced bioethanol. The ultimate objective is still to find the best conditions and technology possible to sustainably and inexpensively produce a high bioethanol yield.